The Spectacular Spider-Man

Josh Keaton

Peter Parker / Spider Man (voice)

Lacey Chabert

Gwen Stacy (voice)

Joshua LeBar

Flash Thompson (voice) / Flash Thompson / Car Thief #1 (voice)

Grey DeLisle

Betty Brant (voice) / Sally Avril (voice) / Sally Avril / Bette Brant (voice) / Sally Avril / Betty Brant (voice) / Sally Avril / Coffee Clerk (voice) / Sally Avril / Computer Voice (voice) / Sally Avril / Erin (voice) / Sally Avril / Voice of OsCorp (voi

Alanna Ubach

Liz Allan (voice) / Liz Allan / Helena (voice) / Liz Allan / Trina (voice)

Vanessa Marshall

Mary Jane Watson (voice) / Mary Jane Watson / Hermia (voice) / Mary Jane Watson / Rosie Thompson (voice)

James Arnold Taylor

Harry Osborn (voice) / Harry Osborn / Alan O'Neil (voice) / Harry Osborn / Frederick Foswell (voice) / Harry Osborn / Patch / Frederick Foswell (voice) / Patch / Homunculi (voice)

Deborah Strang

May Parker (voice)

Clancy Brown

Alex O'Hirn (voice) / Alex O'Hirn / Ox (voice) / Alex O'Hirn / Rhino / George Stacy (voice) / George Stacy (voice) / Rhino / George Stacy (voice)

Alan Rachins

Norman Osborn (voice)

Phil LaMarr

Fancy Dan / Homunculus #1 (voice) / Fancy Dan / Ricochet / Rand Robertson (voice) / Rand Robertson (voice) / Rand Robertson / Fancy Dan (voice) / Rand Robertson / Robbie Robertson (voice) / Robbie Robertson (voice)

Ben Diskin

Eddie Brock (voice) / Eddie Brock / Venom (voice) / Venom (voice)

Daran Norris

J. Jonah Jameson (voice) / J. Jonah Jameson / John Jameson (voice) / J. Jonah Jameson / John Jameson / Colonel Jupiter (voice)

Kevin Michael Richardson

Big Man / Coach Smith / Thug (voice) / Big Man / Tombstone / L. Thompson Lincoln (voice) / Coach Smith (voice) / OsCorp Security Guard (voice) / Principal Davis (voice) / Tombstone (voice) / Tombstone / Principal Davis (voice)

Peter MacNicol

Doctor Octopus (voice) / Master Planner (voice) / Master Planner / Doctor Octopus (voice) / Master Planner / Otto Octavius (voice) / Otto Octavius (voice) / Otto Octavius / Doctor Octopus (voice)

John DiMaggio

Flint Marko / Hammerhead (voice) / Flint Marko / Sandman / Hammerhead (voice) / Hammerhead (voice) / Hammerhead / Car Thief #2 (voice) / Sandman (voice) / Sandman / Hammerhead (voice)

Dee Bradley Baker

Curt Connors (voice) / Curt Connors / Homunculi (voice) / Curt Connors / Lizard (voice)

Cree Summer

Glory Grant (voice) / Glory Grant / Cobweb / Homunculus #2 (voice)

Crispin Freeman

Electro (voice) / Electro / Clerk (voice) / Max Dillon / Electro (voice) / Thug #1 (voice)

Robert Englund

Adrian Toomes / Vulture (voice) / Vulture (voice)

Tricia Helfer

Black Cat (voice) / Felicia Hardy / Black Cat (voice)

Miguel Ferrer

Silvermane (voice) / Silvio Manfredi / Silvermane (voice)

Eric Vesbit

Kraven (voice) / Sergei Kravinoff / Kraven the Hunter (voice)

Ed Asner

Ben Parker (voice)

Danny Trejo

Ox (voice)

Stan Lee

Stan (voice)