Homem-Aranha

Max Mittleman

Harry Osborn (voice)

Scott Menville

Doc Ock / Otto Octavius (voice)

Jim Cummings

Hammerhead / Hammerhead (voice)

Robbie Daymond

Peter Parker / Spider-Man (voice)

Laura Bailey

Gwen Stacy (voice)

Alastair Duncan

Adrian Toomes / Adrian Toomes (voice)

Nadji Jeter

Miles Morales (voice)

Josh Keaton

Norman Osborn / Norman Osborn (voice)

Nancy Linari

Aunt May (voice)

John DiMaggio

Raymond Warren (voice)

Fred Tatasciore

Max Modell (voice)

Melanie Minichino

Anya Corazon (voice)

Ben Diskin

Flash Thompson

Jim Cummings

Hammerhead

Patton Oswalt

Uncle Ben (voice)

Max Mittelman

Harry Osborn (voice)

Jason Spisak

Alistair Smythe (voice) / Alistair Smythe / Scorpion (voice) / Audience Member (voice)

Matthew Mercer

Aleksei Sytsevich / Rhino / Reporter (voice) / Aleksei Sytsevich/Comic Book Guy (voice)

Cameron Boyce

Herman Schultz / Shocker (voice)

Yuri Lowenthal

Clayton Cole / Clash (voice)

Sofia Carson

Keemia (voice) / Sand Girl / Keemia (voice)

Travis Willingham

Sandman / Flint Marko (voice)

Nathaniel J. Potvin

Prowler (voice)

Richard Doyle

Police Officer #3 (voice)

Zeno Robinson

Randy Robertson (voice)

Daniel Hagen

Cop (voice)

Grey DeLisle

Black Cat/Nurse/Mom (voice)