Flash Memory

Neil Patrick Harris

Peter Parker / Spider-Man (voice)

Clancy Brown

Policeman / Raymond

Lisa Loeb

Mary Jane Watson (voice)

Ian Ziering

Harry Osborn (voice)

Angelle Brooks

Indira "Indy" Daimonji (voice)

SuChin Pak

Alison Tomita (voice)

Keith Carradine

J. Jonah Jameson

Michael Dorn

Kraven the Hunter

Virginia Madsen

Silver Sable

Rob Zombie

Dr. Curt Connors / the Lizard

John C. McGinley

Richard Damien

Gina Gershon

Shikata

Cree Summer

Professor Williams / Jolenna

Jennifer Hale

Female Reporter / Female News Announcer

Stan Lee

Frank Elson

Michael Clarke Duncan

Wilson Fisk / The Kingpin (voice)

Karen Maruyama

Shadowy Woman (voice) / Flower Vendor (voice)

Keith David

Agent Mosely (voice)